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KMID : 0613820110210010056
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Journal of Life Science
2011 Volume.21 No. 1 p.56 ~ p.61
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Antioxidant Activities of Ostrich Fern by Different Extraction Methods and Solvents
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Shin So-Lim
Lee Chul-Hee
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Abstract
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This study was performed to investigate effective extract conditions in fronds of the Ostrich fern (Matteuccia struthiopteris) to increase antioxidant compound contents and antioxidant capacity. Powder (1 g) of lyophilizated fronds were mixed with 3 kinds of solvents (MeOH, 80% EtOH and water). Extractions were carried out using not only immersion (room temp.), heating (60℃) and stirring (200 rpm) for 6 hr, but also through sonication in a 42 ㎑ ultrasonic bath for 15, 30 and 45 min. Extracts were filtrated and measured for contents of soluble solids (SS), total polyphenols (TP; tannic acid as a standard) and total flavonoids (TF; Naringin as a standard). Antioxidant activity was expressed as RC50 for DPPH and ABTS radical scavenging. SS (0.317 g·g-1 db), TP (70.90 ㎎·g-1 db) and TF (41.53 ㎎·g-1 db) contents reached their highest levels when 30 minute sonication extraction with 80% EtOH was performed, and the highest DPPH and ABTS scavenging activity was observed in the same extraction conditions (RC50=0.14 ㎎·ml-1 and 0.09 ㎎·ml-1, respectively). From the present investigation, it can be concluded that fronds of the ostrich fern can be used as a natural material for antioxidants, and sonication for 15-30 min with 80% EtOH is an ideal extraction method for increasing their antioxidant effects and saving extraction time.
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KEYWORD
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ABTS radical, DPPH radical, flavonoid, Matteuccia struthiopteris, polyphenol, ultrasonic
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